
Introduction of Microvolume Drop Spectrophotometer
In DNA analysis within the laboratory, determining the concentration and purity of the DNA sample is a crucial step. This serves as a quality control measure, aiding in the decision of whether the sample is suitable for further testing or simply to ascertain its concentration. One of the most used methods to estimate nucleic acid concentration is by measuring sample absorbance at 260 nm. The absorbance ratios 260/280, 260/230, and 260/325 are utilized to determine DNA purity and detect contaminants in biological samples during the DNA extraction process. Currently, the most efficient way to estimate DNA concentration and purity is through absorbance measures of microvolume samples using the microvolume drop spectrophotometer. Since its introduction, this standardized method has gained worldwide adoption.
Overview of the NanoSpec Product
The FastGene® NanoSpec Photometer is a compact microvolume UV-Vis spectrophotometer known for its exceptional user-friendliness. It enables fast and straightforward quantification and analysis of nucleic acids and proteins through UV-Vis absorption spectrophotometry. By offering a robust combination of convenience, precision, and versatility, the FastGene® NanoSpec Photometer stands out as an essential tool for modern laboratory settings, enhancing the efficiency and accuracy of DNA and protein analysis.

Features and Measurement Modes
The FastGene® NanoSpec integrates both microvolume and cuvette measuring modes, allowing the measurement of a wide concentration range. Users can easily set over 20 preconfigured measurement modes, with default values eliminating the need for adjustments. The simple and intuitive menu navigation ensures that measurements are quick, reliable, and easy to perform. The FastGene® NanoSpec Photometer supports fast and accurate quantification and analysis of nucleic acids and proteins through UV-Vis absorption spectrophotometry, covering the full spectrum from 190-850 nm.
Source:
García-Alegría, A. M., Anduro-Corona, I., Pérez-Martínez, C. J., Corella-Madueño, M. A. G., Rascón-Durán, M. L., & Astiazaran-Garcia, H. (2020). Quantification of DNA through the NanoDrop Spectrophotometer: Methodological validation using standard reference material and Sprague Dawley rat and human DNA. International Journal of Analytical Chemistry, 2020, Article ID 8896738, 9 pages.
https://doi.org/10.1155/2020/8896738
https://www.nippongenetics.eu/en/products/lab-instruments/photometer/fastgene-nanospec-photometer/